Combined effect of titanium particles and TNF-alpha on the production of IL-6 by osteoblast-like cells
To clarify the role of tumor necrosis factor (TNF)-alpha on osteoblast functions in the presence of metal particles, two human osteoblast-like cell lines (MG-63 and SaOS-2) were cultured with TNF-alpha in the presence or absence of titanium particles in vitro. A combination of TNF-alpha and titanium particles showed additive effects on inhibition of cell proliferation and alkaline phosphatase production. On the other hand, production of interleukin-6, which is well known to induce osteoclastogenesis and to directly stimulate bone resorption, was additively stimulated by the combination of TNF-alpha and titanium particles. These results suggest that the association of TNF-alpha and titanium particles may play an important role in the pathogenesis of periprosthetic osteolysis through two different pathways: a reduced periprosthetic bone formation due to inhibition of osteoblast proliferation and alkaline phosphatase production, and osteoblast-mediated activation of osteoclastic bone resorption as suggested by the enhancement of interleukin-6 production.
- URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10951379
Keywords: *Biocompatible Materials/adverse effects ; Cell Line ; Humans ; Interleukin-6/*biosynthesis ; Osteoblasts/*drug effects/*metabolism ; Research Support ; Non-U.S. Gov't ; Research Support ; U.S. Gov't ; P.H.S. ; Titanium/*pharmacology ; Tumor Necrosis Factor-alpha/*pharmacology
Department of Orthopaedics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0412, USA.
Record created on 2006-07-25, modified on 2016-08-08