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In this paper, we introduce a framework that merges classical ideas borrowed from scale-space and multi-resolution segmentation with non-linear partial differential equations. A non-linear scale-space stack is constructed by means of an appropriate diffusion equation. This stack is analyzed and a tree of coherent segments is constructed based on relationships between different scale layers. Pruning this tree proves to be a very efficient tool for unsupervised segmentation of different classes of images (e.g. natural, medical ...). This technique is light on the computational point of view and can be extended to non-scalar data in a straightforward manner.