Radiolabeled AOT for studies of the partitioning of the surfactant during protein extraction by means of reverse micellar two-phase systems

Knowledge about the distribution of surfactants between aq. and reverse micellar phases as well as the assocn. of surfactants with proteins are of major importance for assessing the practical feasibility of protein extn. by reverse micellar systems. The question of the fate of the surfactant has been addressed for the classical reverse micellar system based on brine/AOT/isooctane. For this purpose, AOT, (Aerosol OT, di-2-ethyl-1-hexyl sulfosuccinate) was synthesized in the presence of sulfite radioactively labeled with the isotope 35S. The synthesis of [35S]AOT as well as its use for tracer anal. with protein extn. are discussed. [on SciFinder (R)]


Published in:
Journal of Radioanalytical and Nuclear Chemistry, 191, 1, 201-8
Year:
1995
Keywords:
Note:
CAN 123:4970 9-9 Biochemical Methods Inst. Chem. Eng.,Swiss Fed. Inst. Technol.,Lausanne,Switz. Journal 0236-5731 written in English. 163684-74-0P Role: NUU (Other use, unclassified), SPN (Synthetic preparation), PREP (Preparation), USES (Uses) (radiolabeled AOT for studies of partitioning of surfactant during protein extn. by means of reverse micellar two-phase systems); 104-76-7; 108-31-6 (2,5-Furandione); 67161-92-6 Role: RCT (Reactant), RACT (Reactant or reagent) (radiolabeled AOT for studies of partitioning of surfactant during protein extn. by means of reverse micellar two-phase systems); 142-16-5P Role: RCT (Reactant), SPN (Synthetic preparation), PREP (Preparation), RACT (Reactant or reagent) (radiolabeled AOT for studies of partitioning of surfactant during protein extn. by means of reverse micellar two-phase systems); 577-11-7P (Aot) Role: SPN (Synthetic preparation), PREP (Preparation) (radiolabeled AOT for studies of partitioning of surfactant during protein extn. by means of reverse micellar two-phase systems)
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