Process development for the optical resolution of phenylalanine by means of chymotrypsin in a liquid-liquid-solid three-phase reaction system

The stereospecific hydrolysis of phenylalanine esters by chymotrypsin can avantageously be undertaken in aq.-org. 2-phase systems. The partitioning of the deprotonated form of the substrate changes the pH characteristics of the reaction system drastically. It even permits ester hydrolysis to be performed without pH control. Thus, plug flow reactors may be used in the case of immobilized chymotrypsin. However, severe mass transfer limitations are encountered when a fixed bed reactor is fed with an emulsion. [on SciFinder (R)]


Published in:
Biocatalysis in Non-Conventional Media, 163-70
Year:
1992
Publisher:
Amsterdam, Elsevier
Keywords:
Note:
CAN 119:9122 34-2 Amino Acids, Peptides, and Proteins Inst. Genie Chim.,Ec. Polytech. Fed. Lausanne,Lausanne,Switz. Journal 0921-0423 written in English. 9004-07-3 (Chymotrypsin) Role: RCT (Reactant), RACT (Reactant or reagent) (immobilized, as catalyst for resoln. of phenylalanine ester, liq.-liq.-solid flow reactor for); 114260-57-0 (DL-Phenylalanine propyl ester) Role: RCT (Reactant), RACT (Reactant or reagent) (resoln. of, with immobilized chymotrypsin, liq.-liq.-solid flow reactor for)
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 Record created 2006-04-18, last modified 2018-03-17


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