The production of alpha-cyclodextrin by enzymatic degradation of starch

The maximum concentration of alpha-cyclodextrin for the enzymatic degradation of starch is limited to about 13.5 g X 1(-1). By addition of decanol, the equilibrium of the reaction system can be shifted towards an alpha-cyclodextrin yield of 50% even at high substrate concentrations. The main variables of the decanol process--pH, temperature, substrate quality, substrate, and enzyme concentration--have been studied. The cyclodextrin-glycosyltransferase from Klebsiella pneumoniae M5 al can preferentially be employed at pH 6 to 8, temperatures of 40 to 50 degrees C and a decanol concentration of 0.1 kg-1 starch. The dextrose equivalent of starch is important with respect to the maximum achievable starch concentration, but not with respect to the reaction. Under process conditions, the rate of alpha-cyclodextrin evolution is limited by the enzymatic reaction and not by mass transfer of decanol into the aqueous phase. [on SciFinder (R)]


Published in:
Annals of the New York Academy of Sciences, 434, 70-77
Year:
1984
Keywords:
Note:
0077-8923
United States
Journal; Article; (JOURNAL ARTICLE)
English
10016-20-3 (alpha-cyclodextrin); 112-30-1 (n-decyl alcohol); 9005-25-8 (Starch)
Enzyme Engineering 7
Laboratories:




 Record created 2006-04-18, last modified 2018-03-17


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)