000081379 001__ 81379
000081379 005__ 20180318102554.0
000081379 0247_ $$2doi$$a10.1021/bm050016n
000081379 02470 $$2ISI$$a000229138900056
000081379 037__ $$aARTICLE
000081379 245__ $$aProtein-Functionalized Polymer Brushes
000081379 269__ $$a2005
000081379 260__ $$c2005
000081379 336__ $$aJournal Articles
000081379 520__ $$aA new strategy for the prepn. of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymn. (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O6-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O6-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface d. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays. [on SciFinder (R)]
000081379 700__ $$0240028$$aTugulu, Stefano$$g157883
000081379 700__ $$0240322$$aArnold, Anke$$g157870
000081379 700__ $$aSielaff, India
000081379 700__ $$0240057$$aJohnsson, Kai$$g123155
000081379 700__ $$0240029$$aKlok, Harm-Anton$$g156848
000081379 700__ $$0240028$$aTugulu, S.$$g157883
000081379 700__ $$aSielaff, I.
000081379 700__ $$0240029$$aKlok, H.-A.$$g156848
000081379 773__ $$j6$$k3$$q1602-1607$$tBiomacromolecules
000081379 909CO $$ooai:infoscience.tind.io:81379$$pSB$$pSTI$$particle
000081379 909C0 $$0252017$$pLP$$xU10342
000081379 909C0 $$0252027$$pLIP$$xU10102
000081379 937__ $$aLP-ARTICLE-2007-001
000081379 970__ $$a37/LIP
000081379 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000081379 980__ $$aARTICLE