Specific substrates for O6- alkylguanine-DNA alkyltransferase

The invention relates to substrates for O6-alkylguanine-DNA alkyltransferases (AGT) of formula R1-A-X-CH2-R3-R4-L1 (A = a group recognized by AGT as a substrate; X = O, S; R1 = -R2-L2, R5; R2, R4 = linker; R3 = arom. or heteroarom. group, (substituted) unsatd. alkyl, cycloalkyl or heterocyclyl group with the double bond connected to CH2; R5 = arylmethyl, heteroarylmethyl, (substituted) cycloalkyl, cycloalkenyl or heterocyclyl group; L1 = label, plurality of same or different labels, bond connecting R4 to A forming a cyclic substrate, further group -R3-CH2-X-A-R1; L2 = label, plurality of same or different labels). The invention further relates to methods of transferring a label from these substrates to AGT and AGT fusion proteins. [on SciFinder (R)]


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