Methods using O6-alkylguanine-DNA alkyltransferases

A method using O6-alkylguanine-DNA alkyltransferases (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT. This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by attaching mols. to the fusion proteins that introduce a new phys. or chem. property to the fusion protein. Examples of such mols. are, among others, spectroscopic probes or reporter mols., affinity tags, mols. generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or mols. suitable for the immobilization of the fusion protein. A peptide-human AGT fusion protein was labeled in Escherichia coli using I. Biotinylated proteins were detected by Western blotting. [on SciFinder (R)]

(Ecole Polytechnique Federale De Lausanne, Switz.).
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 Record created 2006-02-27, last modified 2018-03-17

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