Modified fibrin hydrogels stimulate angiogenesis in vivo: Potential application to increase perfusion of ischemic tissues

The lack of angiogenesis in ischemic tissues is a major health problem and many studies aim to explore strategies to locally increase blood perfusion. Our approach is to use covalently modified fibrin-based hydrogels as a matrix that induces endothelial cell survival in vitro and angiogenesis in vivo. Fibrin hydrogels were covalently modified by L1Ig6, a specific receptor for cell survival integrin avb3 that is expressed on angiogenic endothelial cells. In addn., L1Ig6-modified matrixes were filled with growth factors VEGF-A165 or bFGF. These hydrogels were applied on growing shell-free chicken chorioallantoic membranes (CAMs) and the developing vasculature was found to be increased by .apprx.50 %. Moreover, the increase in av-integrin levels in the CAMs underlying the hydrogel implants were investigated and found to be increased by .apprx.40 % and .apprx.100 %, resp., after CAM stimulation with L1Ig6 alone or in combination with growth factors VEGF-A165 and bFGF. Therefore, modified fibrin hydrogels provide an interesting way to design an implant that can be introduced at the site of ischemia, and provides a scaffold and release system for growth factors that induce specific tissue responses. [on SciFinder (R)]


Published in:
Materialwissenschaft und Werkstofftechnik, 36, 12, 768-774
Year:
2005
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 Record created 2006-02-27, last modified 2018-01-27


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