A simple solution technique was used to incorporate polyethylene oxide (PEO, of 5000, 10,000, 18,500, and 100,000 g/mol) and other water-soluble polymers such as polyvinylpyrrolidone and polyethyl oxazoline into the surfaces of commonly used biomedical polymers such as polyethylene terephthalate, a polyurethane (Pellethane 2363-80AE), and polymethylmethacrylate. The presence of the water-soluble polymers on these surfaces was verified by using contact angle analysis and ESCA. Protein adsorption studies, fibroblast adhesion assays, and whole blood perfusions over these polymers showed that the surface modified with PEO 18,500 was the most effective in reducing all the tested biological interactions. It was concluded that PEO 18,500 had a chain length that was optimal, using this technique for surface incorporation, to reduce protein adsorption and hence prevent protein-mediated biological interactions. [on SciFinder (R)]