Controlled Immobilization of Membrane Proteins to Surfaces for Fourier Transform Infrared Investigations

We show that it is possible to immobilize membrane proteins uniformly and reversibly as self-assembled (sub)monolayers on nitrilotriacetic acid-covered sensor surfaces via hexahistidine sequences present either in the protein or in lipid membranes. Fourier transform IR spectra of such self-assembled (sub)monolayers deliver important structural information of the membrane proteins and are suited to screen the function of cellular receptors. [on SciFinder (R)]


Published in:
Langmuir, 20, 19, 7901-7903
Year:
2004
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 Record created 2006-02-27, last modified 2018-01-27


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