Nucleic acid sequencing by processive degradation of dye-labeled polynucleotides immobilized on filter membranes

The invention relates to a method and a device for sequencing nucleic acid mols. on a perforated membrane. The inventive method and device can be esp. used in multiplex sequencing. The method involves prepg. an oligonucleotide that is labeled to satn. with base-specific fluorescent dyes that is then immobilized on a filter. The labeled sequence is then processively degraded with an exonuclease with the released bases being detected fluorometrically. [on SciFinder (R)]


Year:
2003
Publisher:
(Gnothis Holding SA, Switz.).
Patent number(s):
Laboratories:




 Record created 2006-02-27, last modified 2018-03-17


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