000081091 001__ 81091
000081091 005__ 20181203020303.0
000081091 0247_ $$2doi$$a10.1002/1522-2683(200205)23:9<1255::AID-ELPS1255>3.0.CO;2-G
000081091 02470 $$2DAR$$a122
000081091 02470 $$2ISI$$a000175693700008
000081091 037__ $$aARTICLE
000081091 245__ $$aImmunoaffinity screening with capillary electrochromatography
000081091 269__ $$a2002
000081091 260__ $$c2002
000081091 336__ $$aJournal Articles
000081091 520__ $$aHighly efficient capillary electrochromatog. sepns. of cardiac glycosides and other steroids are presented. Employing butyl-derivatized silica particles as stationary phase resulted in a nearly three times faster electroosmotic flow (EOF) compared to capillary electrochromatog. (CEC) with octadecyl silica particles. On-column focusing with a preconcn. factor of 180 was performed and sepn. efficiencies of up to 240 000 plates per m were obtained. Using label-free std. UV absorbance, detection limits of 10-80 nM were reached for all steroids tested. For screening of cardiac glycosides, e.g., digoxin and digitoxin in mixts. of steroids, CEC was combined with immunoaffinity extn. using immobilized polyclonal anti-digoxigenin antibodies and Fab fragments. Simply adding small amts. of antibody carrying particles to the samples and comparing chromatograms before and after antibody addn. allowed screening for high affinity antigens in mixts. with moderate nos. of compds. Under conditions of competing antigens, affinity fingerprints of immobilized anti-digoxigenin and anti-digitoxin antibodies were obtained, reflecting the cross-reactivity of eleven steroids. The method provides high selectivity due to the combination of bioaffinity interaction with highly efficient CEC sepn. and UV detection at several wavelengths in parallel. This selectivity was exploited for the detection of four cardiac glycosides in submicromolar concns. in an untreated urine sample. [on SciFinder (R)]
000081091 700__ $$0241728$$g113417$$aMayer, Michael
000081091 700__ $$aMuscate-Magnussen, Angelika
000081091 700__ $$0240645$$g106666$$aVogel, Horst
000081091 700__ $$aEhrat, Markus
000081091 700__ $$aBruin, Gerard J. M.
000081091 773__ $$j23$$tElectrophoresis$$k9$$q1255-1262
000081091 909C0 $$xU10170$$0252153$$pLCPPM
000081091 909CO $$pSB$$particle$$ooai:infoscience.tind.io:81091
000081091 937__ $$aLCPPM-ARTICLE-2002-004
000081091 970__ $$a142/LCPPM
000081091 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000081091 980__ $$aARTICLE