Detection of biomolecules using polymer containing vesicles and functionalized sensors
The invention relates to a functionalized, polymer-reinforced (sterically stabilized) vesicle which comprises a biol., biochem. or synthetic identifying element for identifying and binding a ligand. In addn., the inventive vesicle optionally contains labels which serve as signal-generating constituents in a bioanal. detection method. The invention also relates to a method for producing said vesicle and to the use thereof in detection methods. Thus, self assembled monolayers (SAMs) were produced on the surface of a surface plasmon resonance (SPR) chip, the SAMs were treated with EDC and streptavidin, and functionalized with biotinylated a-Bungarotoxin. Lipid vesicles were produced with DOPC, cholesterol and DOPG, that were stabilized with PEG; nicotinic acetylcholine receptors incorporated. A method is given for the evaluation signals that correspond to non-specific binding. Labels for other detection methods, e.g. ESR, NMR, immunoassay can be added. [on SciFinder (R)]
4199903
Alternative title(s) : (de) Polymer-enthaltendes vesikel und darauf basierte sensor-nachweisverfahren (fr) Vesicule contenant des polymeres et procedes de mise en evidence par detecteur, fondes dessus (en) Vesicle containing polymers and sensor detection methods based thereon
Patent number | Country code | Kind code | Date issued |
JP2003501631 | JP | A | 2003-01-14 |
EP1180242 | EP | A1 | 2002-02-20 |
AU5395000 | AU | A | 2000-12-18 |
WO0073798 | WO | A1 | 2000-12-07 |