Infoscience

Patent

Detection of biomolecules using polymer containing vesicles and functionalized sensors

The invention relates to a functionalized, polymer-reinforced (sterically stabilized) vesicle which comprises a biol., biochem. or synthetic identifying element for identifying and binding a ligand. In addn., the inventive vesicle optionally contains labels which serve as signal-generating constituents in a bioanal. detection method. The invention also relates to a method for producing said vesicle and to the use thereof in detection methods. Thus, self assembled monolayers (SAMs) were produced on the surface of a surface plasmon resonance (SPR) chip, the SAMs were treated with EDC and streptavidin, and functionalized with biotinylated a-Bungarotoxin. Lipid vesicles were produced with DOPC, cholesterol and DOPG, that were stabilized with PEG; nicotinic acetylcholine receptors incorporated. A method is given for the evaluation signals that correspond to non-specific binding. Labels for other detection methods, e.g. ESR, NMR, immunoassay can be added. [on SciFinder (R)]

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