Probing ligand-receptor recognition in G protein-coupled receptors through biosynthetic incorporation of fluorescent amino acids at specific sites and measurement of distances by fluorescence energy transfer

A review with 6 refs. of the authors' work in developing a biophys. technique to investigate the structure, function and dynamics of membrane receptors. A fluorescence-based approach has been developed and applied to the prototypic G protein-coupled receptor NK2. A fluorescent unnatural amino acid was introduced at known sites into NK2 by suppression of nonsense codons with the aid of a chem. misacylated synthetic tRNA specifically designed for the incorporation of unnatural amino acids during expression in Xenopus oocytes. Fluorescence-labeled NK2 mutants contg. a unique fluorescent residue at different sites were investigated by spectrofluorometry in a native membrane environment. Intermol. distances were detd. by measuring the fluorescence resonance energy transfer (FRET) between the fluorescent unnatural amino acid and a fluorescently labeled NK2 ligand. These distances permit one to fix the ligand in space and define the structure of the receptor in a mol. model for NK2 ligand-receptor interactions. [on SciFinder (R)]

Published in:
Fluorescence Microscopy and Fluorescent Probes, 87-92
Presented at:
2nd Conference on Fluorescence Microscopy and Fluorescent Probes, Prague, Apr. 9-12, 1997

 Record created 2006-02-27, last modified 2020-07-30

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