Indirect biomass determination in case of non-constant metabolic rates

A novel approach for indirect biomass detn. in case of non-const. specific metabolic rates is reported. Specific glucose consumption rate was detd. during a first calibration culture for both free suspended and immobilized cells. It was expressed as a linear function of the limiting substrate, i. e. glucose for suspension and oxygen for immobilized cell cultures. For validation, these time-independent models were applied on a second culture presenting a different inoculum cell d. Online monitoring of the limiting substrate enabled a continuous detn. of the specific metabolic rate and thus online cell no. prediction, which was verified with an off-line cell counting technique. [on SciFinder (R)]

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Animal Cell Technology: Products from Cells, Cells as Products, Proceedings of the ESACT Meeting, 16th, Lugano, Switzerland, Apr. 25-29, 1999, 215-217

 Record created 2006-02-27, last modified 2018-03-17

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