Abstract

Continuous cultures of a hybridoma cell line (Zac3) were performed with 2 14C-labeled substrates: glucose and glutamine. A simplified network was used to describe cell metab. This simple network gave a good idea of the main fluxes into the cell, esp. for catabolic reactions. The use of 14C-labeled glucose and glutamine enabled the metabolic routes to be described with great accuracy, esp. CO2 prodn. rate. [on SciFinder (R)]

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