Metabolic network analysis of a hybridoma cell line using mass balances and 14C-labeled glucose and glutamine

Continuous cultures of a hybridoma cell line (Zac3) were performed with 2 14C-labeled substrates: glucose and glutamine. A simplified network was used to describe cell metab. This simple network gave a good idea of the main fluxes into the cell, esp. for catabolic reactions. The use of 14C-labeled glucose and glutamine enabled the metabolic routes to be described with great accuracy, esp. CO2 prodn. rate. [on SciFinder (R)]

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New Developments and New Applications in Animal Cell Technology, Proceedings of the ESACT Meeting, 15th, Tours, Fr., Sept. 1997, 351-353

 Record created 2006-02-27, last modified 2018-03-17

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