Comparison of different bioreactors and media for the production of specific monoclonal IgA antibodies

An IgA producing hybridoma cell line has been grown in various bioreactor systems, in serum-contg. and serum-free media. Bioreactors and media have been compared on the basis of product quantity (total IgA) and quality (activity, monomer/dimer/polymer ratios). IgA was produced in vitro with an activity similar to ascites fluid. The serum-free media was found to be better for such purposes. Prodn. in hollow fiber reactors resulted in high concns. of product, but with a lower activity fraction, possibly due to the formation of aggregates. [on SciFinder (R)]

Published in:
Animal Cell Technology: Developments towards the 21st Century, [Proceedings of the Meeting], Veldhoven, Neth., Sept. 12-16, 1994, 495-499

 Record created 2006-02-27, last modified 2018-03-17

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