000080652 001__ 80652
000080652 005__ 20180127194136.0
000080652 02470 $$2DAR$$a153
000080652 02470 $$2ISI$$a000176150100020
000080652 037__ $$aARTICLE
000080652 245__ $$a3'-Deoxyribopyranose (4' -> 2')-oligonucleotide duplexes (=p-DNA duplexes) as substitutes of RNA hairpin structures
000080652 260__ $$c2002
000080652 269__ $$a2002
000080652 336__ $$aJournal Articles
000080652 520__ $$aBy automated synthesis, we prepd. hybrid oligonucleotides consisting of covalently linked RNA and p-DNA sequences (p-DNA = 3'-deoxyribopyranose (4'->2')-oligonucleotides). The pairing properties of corresponding hybrid duplexes formed from fully complementary single strands were investigated. An uninterrupted p-p-stacking at the p-DNA/RNA interface and cooperative pairing between the two systems was achieved by connecting them via a 4'-p-DNA-2'->5'-RNA-3' and 5'-RNA-2'->4'-p-DNA-2' phosphodiester linkage, resp. The RNA 2'-phosphoramidites required for the formation of the RNA-2'->4'-p-DNA phosphodiester linkage were synthesized from the corresponding, 3'-O-tom-protected ribonucleosides (tom = [(triisopropylsilyl)oxy]methyl). Analogs of the FMN binding aptamer and the hammerhead ribozyme were prepd. Each of these analogs consisted of two p-DNA/RNA hybrid single strands with complementary p-DNA sequences, designed to substitute stem/loop and stem motifs within the parent compds. By comparative binding and cleavage studies, it was found that mixing of the two complementary p-DNA/RNA hybrid sequences resulted in the formation of the fully functional analogs of the FMN-binding aptamer and of the hammerhead ribozyme, resp. [on SciFinder (R)]
000080652 700__ $$aAckermann, Damian
000080652 700__ $$aWu, Xiaolin
000080652 700__ $$0240732$$aPitsch, Stefan$$g145837
000080652 773__ $$j85$$k5$$q1463-1478$$tHelvetica Chimica Acta
000080652 909C0 $$0252378$$pLCAN
000080652 909CO $$ooai:infoscience.tind.io:80652$$particle
000080652 937__ $$aLCAN-ARTICLE-2002-002
000080652 970__ $$a13/LCAN
000080652 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000080652 980__ $$aARTICLE