Tilt Angle Dependence of Cross-Relaxation in Off-Resonance ROESY

We present an efficient exptl. method to evaluate whether the effective cross-relaxation rate between a pair of spins vanishes when applying an off-resonance spin-lock field. It is shown that the cross-relaxation rate can be made to vanish even when the two spins concerned resonate at different offsets and experience significantly different tilt angles of their resp. spin-lock fields. This is verified exptl. using a sample of 15N-labeled human ubiquitin, through selective excitation of chosen amide protons. The results are relevant for the quant. interpretation of off-resonance ROESY expts. (c) 1999 Academic Press. [on SciFinder (R)]

Published in:
Journal of Magnetic Resonance, 138, 2, 326-329

 Record created 2006-02-22, last modified 2018-03-17

Rate this document:

Rate this document:
(Not yet reviewed)