Normalized one-dimensional NOE measurements in isotopically labeled macromolecules using two-way cross-polarization

A novel one-dimensional NOE expt. is presented where a selected proton is excited by two-way heteronuclear cross-polarization between protons and nitrogen-15 or carbon-13. The utility of the method is demonstrated for a sample of 15N labeled human ubiquitin. Inter- and intra-residue NOEs are clearly obsd. in a very time-effective manner. The signal intensities can be easily normalized. [on SciFinder (R)]


Published in:
Journal of Biomolecular NMR, 13, 1, 61-65
Year:
1999
Laboratories:




 Record created 2006-02-22, last modified 2018-03-17


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