000080376 001__ 80376
000080376 005__ 20180317093603.0
000080376 037__ $$aARTICLE
000080376 245__ $$aMeasurement of relaxation rates of N(H) and H(alpha) backbone protons in proteins with tailored initial conditions
000080376 269__ $$a1999
000080376 260__ $$c1999
000080376 336__ $$aJournal Articles
000080376 520__ $$aSeveral methods are presented for the selective determination of spin-lattice and spin-spin relaxation rates of backbone protons in labeled proteins. The relaxation rates of amide protons in (15)N labeled proteins can be measured by using two-way selective cross-polarization (SCP). The measurement of H(alpha) relaxation rates can be achieved by combining this method with homonuclear Hartmann-Hahn transfer using doubly selective irradiation. Various schemes for selective or nonselective inversion of the longitudinal proton magnetization lead to different initial recovery rates. The methods have been applied to lysine K6 in (15)N-labeled human ubiquitin and to leucine L5 in (15)N- and (13)C-labeled octapeptide YG*G*F*LRRI (GFL) in which the marked residues are (15)N- and (13)C-labeled. Copyright 1999 Academic Press. [on SciFinder (R)]
000080376 700__ $$aMillet, O.
000080376 700__ $$aChiarparin, E.
000080376 700__ $$0240607$$aPelupessy, P.$$g166916
000080376 700__ $$aPons, M.
000080376 700__ $$0243177$$aBodenhausen, G.$$g122795
000080376 773__ $$j139$$k2$$q434-8$$tJournal of magnetic resonance
000080376 909CO $$ooai:infoscience.tind.io:80376$$particle$$pSB
000080376 909C0 $$0252152$$pLRMB$$xU10098
000080376 937__ $$aLRMB-ARTICLE-1999-010
000080376 970__ $$a243/LRMB
000080376 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000080376 980__ $$aARTICLE