Some preliminary results on the action of rhamnogalacturonase on rhamnogalacturonan oligosaccharides from beet pulp

Sugar-beet pulp was saponified and then hydrolysed with 0.1 M HCl at 80 degrees C for 72 h, and a rhamnogalacturonan fraction was isolated by ion-exchange chromatography on AG 1X8 resin. Four individual oligomers, and a mixture of oligomers with higher degrees of polymerization, were obtained by chromatography on BioGel P-4. They all presented the alpha-D-GalAp-(1[-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GalAp-(1](n)-->2)-L- Rhap structure (with n greater than or equal to 2) The five fractions were submitted to hydrolysis with rhamnogalacturonase. The enzyme was active on oligomers with degrees of polymerization greater than or equal to 10, and gave as main products alpha-L-Rhap-(1-->4)-alpha-D-GalAp-(1-->2)-or-L-Rhap(1-->4)-D-GalAp and alpha-D-GalAp-(1-->2)-alpha-L-Rhap-(1-->4)-alpha-D-GalAp-(1-->2)-alpha-L -Rhap-(1-->4)-D-GalAp.


Published in:
Int. J. Biol. Macromol., 17, 6, 333-336
Year:
1995
ISSN:
0141-8130
Laboratories:




 Record created 2006-02-09, last modified 2018-01-27


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)