ANALYSIS OF SYNTHETIC PEPTIDES USING MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY

Matrix-assisted laser desorption ionization mass spectroscopy (LDI MS), a novel method for analysis of large molecules, has been used for characterization of synthetic peptides and their by-products. The potential of LDI MS is demonstrated by analyzing crude synthetic peptides representing typical members of newly designed peptides and proteins. In the first case, a fragment condensation reaction yielding a highly hydrophobic six-helix bundle template-assembled synthetic protein (TASP) is monitored. Then, a crude 19-mer peptide designed to adopt an amphiphilic alpha-helical structure and its by-products from SPPS are identified. Finally, analysis of crude hirulog-1, a 20-mer peptide designed as a thrombin inhibitor, using C18 reversed phase high performance liquid chromatography (RP HPLC), capillary electrophoresis (CE) ahd LDI MS, manifests the potential of the latter method.


Published in:
Peptide Research, 5, 1, 25-29
Year:
1992
Laboratories:




 Record created 2006-02-09, last modified 2018-01-27


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