The Micro Liquid/Liquid Interface as a Transducer for Creatinine Assay

Facilitated ammonium ion transfer reactions, using the crown ether dibenzo-18-crown-6, at a single microhole supported water/1,2-dichloroethane interface were used as the transducer to detect the biomolecule creatinine. The ammonium ion was produced during the enzymatic action of creatinine deiminase on creatinine. A prototype sensing device incorporating a Teflon gas permeable membrane, to exclude possible interferents, yielded a linear amperometric response to added creatinine from 20 uM to 1 mM. This range is sufficient for that required for the determination of creatinine in serum.


Published in:
Microchimica Acta, 117, 3-4, 175-185
Year:
1995
Laboratories:




 Record created 2005-11-07, last modified 2018-12-03


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