Pumping of mammalian cells with a nozzle-diffuser micropump

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.


Publié dans:
Lab On a Chip, 5, 10, 1083-1088
Année
2005
Publisher:
Royal Society of Chemistry
ISSN:
1473-0197
Mots-clefs:
Laboratoires:




 Notice créée le 2005-10-26, modifiée le 2019-03-16

Publisher's version:
Télécharger le document
PDF

Évaluer ce document:

Rate this document:
1
2
3
 
(Pas encore évalué)