000055891 001__ 55891
000055891 005__ 20190316233547.0
000055891 0247_ $$2doi$$a10.1039/b504468e
000055891 022__ $$a1473-0197
000055891 02470 $$2ISI$$a000232003200012
000055891 037__ $$aARTICLE
000055891 245__ $$aPumping of mammalian cells with a nozzle-diffuser micropump
000055891 269__ $$a2005
000055891 260__ $$bRoyal Society of Chemistry$$c2005
000055891 336__ $$aJournal Articles
000055891 520__ $$aWe discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.
000055891 6531_ $$aJurkat cells
000055891 6531_ $$aHybridoma cells
000055891 6531_ $$aLab-On-a-Chip
000055891 6531_ $$aMicropump
000055891 6531_ $$aValve-less
000055891 6531_ $$aNozzle
000055891 6531_ $$aDiffuser
000055891 700__ $$0241365$$g104278$$aYamahata, C.
000055891 700__ $$0240440$$g138412$$aVandevyver, C.
000055891 700__ $$0241033$$g156714$$aLacharme, F.
000055891 700__ $$0241077$$g149686$$aIzewska, P.
000055891 700__ $$0240645$$g106666$$aVogel, H.
000055891 700__ $$g110869$$aFreitag, R.$$0241367
000055891 700__ $$0242801$$g113762$$aGijs, M. A. M.
000055891 773__ $$j5$$tLab On a Chip$$k10$$q1083-1088
000055891 8564_ $$uhttps://infoscience.epfl.ch/record/55891/files/b504468e.pdf$$zn/a$$s457801$$yPublisher's version
000055891 909C0 $$xU10322$$0252094$$pLMIS2
000055891 909C0 $$0252153$$pLCPPM$$xU10170
000055891 909CO $$qGLOBAL_SET$$pSB$$pSTI$$particle$$ooai:infoscience.tind.io:55891
000055891 917Z8 $$x104278
000055891 937__ $$aLMIS2-ARTICLE-2005-007
000055891 970__ $$a145/LMIS2
000055891 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000055891 980__ $$aARTICLE