000054098 001__ 54098
000054098 005__ 20190316233523.0
000054098 0247_ $$2doi$$a10.1039/b417604a
000054098 02470 $$2DAR$$a6803
000054098 02470 $$2ISI$$a000228719100011
000054098 037__ $$aARTICLE
000054098 245__ $$aGentle cell trapping and release on a microfluidic chip by in situ alginate hydrogel formation
000054098 269__ $$a2005
000054098 260__ $$c2005
000054098 336__ $$aJournal Articles
000054098 520__ $$aMicrofluidic devices are increasingly used to perform biological experiments on a single-cell basis. However, long-term stability of cell positions is still an issue. A novel biocompatible method for cell entrapment and release on a microchip is presented. It is based on the controlled formation of an alginate hydrogel by bringing two laminar flows of alginate and calcium ions in the range of 2 mM to 40 mM into contact. The resulting growth of a gel bar is used to enclose and immobilize yeast cells. Adding ethylenediaminetetraacetic acid ( EDTA) to the alginate solution allows for control of the hydrogel growth, and by varying the ratio of Ca2+ to EDTA concentrations gel growth or gel shrinkage can be induced at will. Trapped cells are released during shrinkage of the gel. The trapping efficiency for different cell speeds is investigated and the properties of gel growth are discussed using a diffusion model. Precise positioning of a single cell is demonstrated. The technique presented allows not only the reversible immobilization of cells under gentle conditions but also offers the potential of long-term cell cultures as shown by on-chip incubation of yeast cells. The procedure may provide a simple and fully biocompatible technique for a multitude of innovative experiments on cells in microsystems.
000054098 6531_ $$asoft lithography
000054098 6531_ $$amicrostructures
000054098 6531_ $$adevices
000054098 6531_ $$aarrays
000054098 700__ $$0240218$$g157067$$aBraschler, Thomas
000054098 700__ $$0241378$$g152134$$aJohann, Robert
000054098 700__ $$aHeule, Martin
000054098 700__ $$0240718$$g136851$$aMetref, Lynda
000054098 700__ $$0240219$$g107144$$aRenaud, Philippe
000054098 773__ $$j5$$tLAB ON A CHIP$$k5$$q553-559
000054098 8564_ $$zURL
000054098 8564_ $$uhttps://infoscience.epfl.ch/record/54098/files/Braschler%2705-LabChip.pdf$$zn/a$$s366581
000054098 909C0 $$xU10324$$0252064$$pLMIS4
000054098 909CO $$ooai:infoscience.tind.io:54098$$qGLOBAL_SET$$pSTI$$particle
000054098 937__ $$aLMIS4-ARTICLE-2005-003
000054098 970__ $$aISI:000228719100011/LMIS4
000054098 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000054098 980__ $$aARTICLE