000053746 001__ 53746
000053746 005__ 20181203015831.0
000053746 022__ $$a1525-0016
000053746 0247_ $$2doi$$a10.1006/mthe.2002.0653
000053746 037__ $$aARTICLE
000053746 245__ $$aHighly efficient lentiviral vector-mediated transduction of nondividing, fully reimplantable primary hepatocytes
000053746 269__ $$a2002
000053746 260__ $$c2002
000053746 336__ $$aJournal Articles
000053746 520__ $$aGene therapy is an attractive approach for the treatment of liver disease. We demonstrate that a so-called third-generation human immunodeficiency virus (HIV)-derived vector system can govern the efficient delivery, integration, and stable expression of a transgene into primary human hepatocytes in the complete absence of cell division. We also show that rodent hepatocytes exhibit a significant degree of resistance to HIV vector-mediated transduction, a phenotype that is particularly pronounced in murine hepatocytes and that results from a block in the immediate-early phase of infection. We finally describe a methodology, that allows very high rates of transduction through minimal in vitro manipulation, in which hepatocytes are kept in suspension and reimplanted within a few hours of harvest with a fully preserved engraftment potential. These results have immediate implications for the treatment of liver diseases by the transplantation of genetically modified hepatocytes, an approach that could be applied to a number of hereditary and acquired hepatic disorders.
000053746 6531_ $$aGenetic Vectors
000053746 6531_ $$aTransduction
000053746 6531_ $$aGenetic
000053746 700__ $$aNguyen, Tuan Huy
000053746 700__ $$aOberholzer, José
000053746 700__ $$aBirraux, Jacques
000053746 700__ $$aMajno, Pietro
000053746 700__ $$aMorel, Philippe
000053746 700__ $$g167919$$aTrono, Didier$$0240083
000053746 773__ $$j6$$tMol Ther$$k2$$q199-209
000053746 909C0 $$xU11172$$0252036$$pLVG
000053746 909CO $$pSV$$particle$$ooai:infoscience.tind.io:53746
000053746 937__ $$aLVG-ARTICLE-2002-006
000053746 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000053746 970__ $$a12161186/LVG
000053746 980__ $$aARTICLE