000053705 001__ 53705
000053705 005__ 20190527124957.0
000053705 022__ $$a0022-538X
000053705 037__ $$aARTICLE
000053705 245__ $$aSelf-inactivating lentivirus vector for safe and efficient in vivo gene delivery
000053705 269__ $$a1998
000053705 260__ $$bAmerican Society for Microbiology$$c1998
000053705 336__ $$aJournal Articles
000053705 520__ $$aIn vivo transduction of nondividing cells by human immunodeficiency virus type 1 (HIV-1)-based vectors results in transgene expression that is stable over several months. However, the use of HIV-1 vectors raises concerns about their safety. Here we describe a self-inactivating HIV-1 vector with a 400-nucleotide deletion in the 3' long terminal repeat (LTR). The deletion, which includes the TATA box, abolished the LTR promoter activity but did not affect vector titers or transgene expression in vitro. The self-inactivating vector transduced neurons in vivo as efficiently as a vector with full-length LTRs. The inactivation design achieved in this work improves significantly the biosafety of HIV-derived vectors, as it reduces the likelihood that replication-competent retroviruses will originate in the vector producer and target cells, and hampers recombination with wild-type HIV in an infected host. Moreover, it improves the potential performance of the vector by removing LTR sequences previously associated with transcriptional interference and suppression in vivo and by allowing the construction of more-stringent tissue-specific or regulatable vectors.
000053705 6531_ $$aGenetic Vectors
000053705 700__ $$0240510$$g162095$$aZufferey, R.
000053705 700__ $$aDull, T.
000053705 700__ $$aMandel, R. J.
000053705 700__ $$aBukovsky, A.
000053705 700__ $$aQuiroz, D.
000053705 700__ $$aNaldini, L.
000053705 700__ $$0240083$$g167919$$aTrono, Didier
000053705 773__ $$j72$$tJournal of Virology$$k12$$q9873-80
000053705 909C0 $$xU11172$$0252036$$pLVG
000053705 909C0 $$xU10457$$0252067$$pLEN
000053705 909CO $$pSV$$particle$$ooai:infoscience.tind.io:53705
000053705 917Z8 $$x182396
000053705 937__ $$aLVG-ARTICLE-1998-005
000053705 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000053705 970__ $$a9811723/LVG
000053705 980__ $$aARTICLE