In situ observations of biophysical interactions in natural waters typically focus on physical mechanisms influencing biological activity. Yet, biological activity can also drive physical processes in aquatic environments. A community of photoautotrophic, motile and heavy bacteria—Chromatium okenii, which requires light, sulfide, and anoxic conditions to perform anoxygenic photosynthesis, accumulates below the chemocline of the meromictic Lake Cadagno (Switzerland). Upward vertical migration drives bioconvection, which modifies the physical environment of the bacteria-populated water to create a deep and homogeneous mixed layer of up to 1 m thickness. Continuous convection within the mixed layer and diapycnal diffusivity from its adjacent stratified surroundings determine ecologically relevant gradients. The daytime vertical migration that induce convective instabilities is well-established. It consists in bacteria swimming upward towards light and accumulating at the upper part of the anoxic layer, leading to a locally-unstable density excess. However, nocturnal activity has not yet been analyzed. An intensive 48-h survey was conducted in August 2018 using standard and microstructure profilers, as well as a moored high-resolution current meter coupled with temperature and turbidity sensors deployed across the mixed layer depth. This survey revealed a persistent mixed layer also during nighttime hours. Using a mixed layer shape model, vertical velocity observations and turbulent dissipation estimates, we conclude that photoautotrophic bacteria continue their vertical migration at night. This nocturnal activity thereby drives “dark bioconvection” and maintains the subsurface mixed bacterial layer in Lake Cadagno throughout the diel cycle.