C-13 Dynamic Nuclear Polarization using SA-BDPA at 6.7 T and 1.1 K: Coexistence of Pure Thermal Mixing and Well-Resolved Solid Effect

SA-BDPA is a water-soluble, narrow-line width radical previously used for dynamic nuclear polarization (DNP) signal enhancement in solid-state magic angle spinning NMR spectroscopy. Here, we report the first study using SA-BDPA under dissolution DNP conditions (6.7 T and 1.15 K). Longitudinal-detected (LOD)-electron spin resonance (ESR) and C-13 DNP measurements were performed on samples containing 8.4 M [C-13]urea dissolved in 50:50 water:glycerol (v/v) doped with either 60 or 120 mM SA-BDPA. Two distinct DNP mechanisms, both "pure" thermal mixing and a well-resolved solid effect could clearly be identified. The radical's ESR line width (30-40 MHz), broadened predominantly by dipolar coupling, excluded any contribution from the cross effect. Microwave frequency modulation increased the enhancement by DNP at the lower radical concentration but not at the higher radical concentration. These results are compared to data acquired with trityl radical AH111501, highlighting the unusual C-13 DNP properties of SA-BDPA.


Published in:
Journal Of Physical Chemistry Letters, 11, 16, 6873-6879
Year:
Aug 20 2020
Publisher:
Washington, AMER CHEMICAL SOC
ISSN:
1948-7185
Keywords:
Laboratories:




 Record created 2020-09-13, last modified 2020-10-29


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)