Generalized temporal sampling with active illumination in optical microscopy

Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overlapping inner-products and because only positive signals (intensities) can be measured by cameras. We present a method to collect videos of monochromatic objects by projecting the incoming signal at each pixel in a temporal B-spline space of degree 0, 1, or 2 by using a conventional RGB camera and a modulated three-color light source for illumination. Specifically, we solve the basis function overlap problem by multiplexing the acquisition in different color ranges and use B-spline pieces (which are positive) as projection kernels of a biorthogonal projection-expansion bases pair. The steps to recover signal samples include spectral unmixing and inverse filtering. Reconstructions we obtained from simulated and experimentally-acquired microscopy data demonstrate the feasibility of our approach.


Published in:
11138
Presented at:
SPIE - Proceeding of the SPIE Conference Optics and Photonics, Wavelets and Sparsity XVIII, San Diego, California, United States
Year:
2019
Publisher:
SPIE
Keywords:
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 Record created 2019-09-05, last modified 2019-09-17

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