Imaging Lipid Metabolism at the Golgi Complex

The development of fluorescence-based molecular imaging has revolutionized cell biology allowing the visualization of specific biomolecules at the microscopic and, more recently, at the nanoscopic scale while in their relevant biological contexts. Nonetheless, despite the imaging toolkit for biologists interested in exploring the subcellular localization and dynamics of proteins and nucleic acids has expanded exponentially in the last decades, the means to visualize and track lipids in cells did not develop to the same extent until recently. Here we described some basic fluorescence-based techniques that can be used in standard cell biology laboratories to visualize subcellular pools of specific lipids and to evaluate their regional metabolism. Specifically, here we focus on the imaging-based analysis of phosphoinositide and sphingolipid metabolism at the Golgi complex.


Published in:
Intracellular Lipid Transport: Methods and Protocols, 47-56
Year:
Feb 22 2019
Publisher:
Springer Science+Business Media
ISBN:
978-1-4939-9135-8
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2019-04-16, last modified 2019-06-16

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