000264976 001__ 264976
000264976 005__ 20190619220214.0
000264976 037__ $$aPOST_TALK
000264976 245__ $$aRDHK Family, Regulators dedicated to Organohalide Respiration - Sequence Diversity and Functional Prediction
000264976 260__ $$c2019-02-01
000264976 269__ $$a2019-02-01
000264976 336__ $$aPosters
000264976 513__ $$aPosters
000264976 520__ $$aOrganohalide respiration (OHR) is an anaerobic metabolism by which bacteria conserve energy from the use of organohalide molecules as terminal electron acceptors. Because most organohalides of anthropogenic origin are persistent pollutants, the study of bacteria capable of OHR (OHRB) has a strong environmental interest. OHRB appear in three major phyla (Firmicutes, Proteobacteria and Chloroflexi) and encode at least one reductive dehalogenase (RdhA) in their genomes. RdhA enzymes are the key catalytic subunit in OHR and their corresponding genes are often surrounded by accessory genes organized in rdh gene clusters. Among them, rdhK is coding for a member of the CRP/FNR superfamily of transcriptional regulators and is dedicated to the regulation of rdh genes in Firmicutes. Upon binding of an effector molecule (i.e. organohalide) the regulator recognizes a specific DNA motif in the promotor region of the target genes in order to recruit the RNA polymerase and activate transcription. So far, based on the characterization of only three RdhK proteins, it was assumed that one RdhK regulator senses specific organohalide compounds which are the substrates of the RdhA enzyme encoded in the same rdh gene cluster. Therefore, the identification of the binding partners (both effector molecule and target DNA sequence) for each new RdhK regulator can be used as an indirect way to define potential substrates of yet uncharacterized RdhA enzymes. Firstly, we aimed to study the diversity of RdhK regulators in Firmicutes. In this regard, BLAST search was used to identify CRP/FNR regulators encoded by genes located in rdhA direct vicinity in the available genome sequences of Dehalobacter spp. and Desulfitobacterium spp. This resulted in a list of 96 RdhK sequences that will be used for sequence similarity analysis in order to define RdhK subgroups. Moreover, a tentative identification of signature motifs will be done through the analysis of individual subgroups. Finally, our RdhK sequence database will be confronted to the available knowledge on organohalide specificity of characterized RdhA enzymes. This should help predicting the range of effectors and substrates for pairs of RdhK regulators and RdhA enzymes, respectively. Such correspondence could possibly lead to a common type of effectors for a given subgroup of RdhK regulators which would have to be verified through biochemical work.
000264976 700__ $$g276106$$aWillemin, Mathilde Stéphanie$$0250206
000264976 700__ $$g121849$$aHolliger, Christof$$0240405
000264976 700__ $$0241604$$aMaillard, Julien$$g138380
000264976 7112_ $$aSwiss Microbial Ecology Meeting - SME 2019$$cLausanne, Switzerland$$d30 Jan to 1 Feb 2019
000264976 8560_ $$ffilomena.jacquier@epfl.ch
000264976 8564_ $$uhttps://infoscience.epfl.ch/record/264976/files/FinalVersion_SME_MW.pdf$$s3603294
000264976 8564_ $$uhttps://infoscience.epfl.ch/record/264976/files/MW_FinalAbstract.pdf$$s42416
000264976 909C0 $$zCharbonnier, Valérie$$xU10268$$pLBE$$mchristof.holliger@epfl.ch$$0252125
000264976 909CO $$ppresentation$$pENAC$$ooai:infoscience.epfl.ch:264976$$pposter
000264976 960__ $$afilomena.jacquier@epfl.ch
000264976 961__ $$afantin.reichler@epfl.ch
000264976 973__ $$aEPFL$$sPUBLISHED
000264976 980__ $$aPOST_TALK
000264976 981__ $$aoverwrite