000264873 001__ 264873
000264873 005__ 20190319155151.0
000264873 0247_ $$2doi$$a10.1021/acs.analchem.9b00659
000264873 02470 $$a10.1021/acs.analchem.9b00659$$2DOI
000264873 037__ $$aARTICLE
000264873 245__ $$aCombining Ultrahigh-Resolution Ion-Mobility Spectrometry with Cryogenic Infrared Spectroscopy for the Analysis of Glycan Mixtures
000264873 260__ $$c2019
000264873 269__ $$a2019
000264873 336__ $$aJournal Articles
000264873 520__ $$aThe isomeric complexity of glycans make their analysis by traditional techniques particularly challenging. While the recent combination of ion mobility spectrometry (IMS) with cryogenic IR spectroscopy has demonstrated promise as a new tech-nique for glycan analysis, this approach has been limited by the modest resolution of the ion mobility stage. In this work we report results from a newly developed instrument that combines ultrahigh-resolution IMS with cryogenic IR spectroscopy for glycan analysis. This apparatus makes use of the recent development in travelling-wave IMS called structures for lossless ion manipulation (SLIM). The IMS stage allows the selection of glycan isomers that differ in collisional cross section by as little as 0.2% before injecting them into a cryogenic ion trap for IR spectral analysis. We compare our results to those using drift-tube IMS and highlight the advantages of the substantial increase in resolution. Application of this approach to glycan mixtures demonstrates our ability to isolate individual components, measure a cryogenic IR spectrum, and identify them using a spectroscopic database.
000264873 536__ $$aFNS$$c200020_165908
000264873 6531_ $$amass spectrometry
000264873 6531_ $$aion mobility
000264873 6531_ $$aion spectroscopy
000264873 6531_ $$avibrational spectroscopy
000264873 700__ $$aBen Faleh, Ahmed
000264873 700__ $$aWarnke, Stephan
000264873 700__ $$aRizzo, Thomas R.
000264873 773__ $$tAnalytical Chemistry
000264873 8560_ $$fthomas.rizzo@epfl.ch
000264873 909C0 $$zBorel, Alain$$xU10106$$pLCPM$$mthomas.rizzo@epfl.ch$$0252096
000264873 909CO $$ooai:infoscience.epfl.ch:264873$$particle$$pSB
000264873 960__ $$athomas.rizzo@epfl.ch
000264873 961__ $$afantin.reichler@epfl.ch
000264873 973__ $$aEPFL$$sPUBLISHED$$rREVIEWED
000264873 980__ $$aARTICLE
000264873 981__ $$aoverwrite