000264517 001__ 264517
000264517 005__ 20190325124401.0
000264517 022__ $$a2161-5063
000264517 02470 $$a000459367000027$$2isi
000264517 0247_ $$a10.1021/acssynbio.8b00427$$2doi
000264517 037__ $$aARTICLE
000264517 245__ $$aA Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System
000264517 260__ $$c2019$$aWashington$$bAMER CHEMICAL SOC
000264517 269__ $$a2019-02-01
000264517 336__ $$aJournal Articles
000264517 520__ $$aWe demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The one pot method makes the PURE system easy to generate and allows it to be readily optimized and modified.
000264517 650__ $$aBiochemical Research Methods
000264517 650__ $$aBiochemistry & Molecular Biology
000264517 6531_ $$asynthetic biology
000264517 6531_ $$aprotein expression
000264517 6531_ $$acell-free transcription and translation
000264517 6531_ $$apure system
000264517 6531_ $$aprinciples
000264517 700__ $$aLavickova, Barbora$$0250333
000264517 700__ $$aMaerkl, Sebastian J.$$0243949
000264517 773__ $$k2$$j8$$q455-462$$tAcs Synthetic Biology
000264517 8560_ $$fsebastian.maerkl@epfl.ch
000264517 909C0 $$zMarselli, Béatrice$$0252240$$yApproved$$pLBNC$$xU11835$$msebastian.maerkl@epfl.ch
000264517 909CO $$particle$$ooai:infoscience.epfl.ch:264517$$pSTI
000264517 961__ $$apierre.devaud@epfl.ch
000264517 973__ $$aEPFL$$sPUBLISHED$$rREVIEWED
000264517 980__ $$aARTICLE
000264517 980__ $$aWoS
000264517 981__ $$aoverwrite