Imaging cellular ultrastructures using expansion microscopy (U-ExM)

Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.


Published in:
Nature Methods, 16, 1, 71-74
Year:
Jan 01 2019
ISSN:
1548-7091
1548-7105
Keywords:
Laboratories:




 Record created 2019-01-23, last modified 2019-06-25

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