High-throughput multiplexed fluorescence-activated droplet sorting

Fluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous populations. Our experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.


Published in:
Microsystems & Nanoengineering, 4, UNSP 33
Year:
Oct 22 2018
Publisher:
London, NATURE PUBLISHING GROUP
ISSN:
2055-7434
Keywords:
Laboratories:




 Record created 2018-12-13, last modified 2019-01-22


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