Multicolor single-particle reconstruction of protein complexes

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.


Publié dans:
Nature Methods, 15, 10, 777-+
Année
Oct 01 2018
ISSN:
1548-7091
1548-7105
Mots-clefs:
Laboratoires:




 Notice créée le 2018-12-13, modifiée le 2019-06-19


Évaluer ce document:

Rate this document:
1
2
3
 
(Pas encore évalué)