Motor responses in hindlimb muscles to epidural spinal cord stimulation in normal awake rats during bipedal standing were studied. Stimulation at L2 or S1 induced simultaneous and bilateral responses in the vastus lateralis, semitendinosus, tibialis anterior, and medial gastrocnemius muscles. Stimulation at S1 evoked an early (ER), middle (MR) and late (LR) response: stimulation at L2 elicited only a MR and LR. Vibration and double epidural stimulation testing suggests that the ER is a direct motor response, whereas the MR and LR are mediated synaptically. MR has properties of a monosynaptic reflex, i.e., inhibited during vibration and depressed during the second pulse of a double stimulation. Some components of the LR seem to be mediated by afferents associated with the flexor reflex and probably involve group II afferents. During bipedal treadmill stepping, the MR was modulated in extensors, whereas the LR was modulated in flexors. These results show differential modulation of monosynaptic and polysynaptic reflexes in flexor and extensor motor pools during locomotion. Monosynaptic responses to stimulation at either L2 or S1 generally were amplified in extensors during the stance phase and in flexors during the swing phase of the step cycle. No correlation was found between the ER and the EMG background during stepping, whereas both the MR and LR were closely correlated with the changes in the EMG activity level of the corresponding muscle. These data demonstrate the feasibility of using epidural stimulation for examining monosynaptic and polysynaptic pathways to motor pools associated with multiple muscles during movement and over a prolonged period