Bond behavior and anchorage resistance of prestressed CFRP plates to steel substrate have been investigated in the current study. To do so, a set of single lap-shear and prestress release tests was performed on CFRP-to-steel bonded joints, and the feasibility of accelerated curing (AC) of epoxy adhesive by heating was investigated. Furthermore, a set of so called mixed bond tests, with partial prestress release and subsequent lap-shear, was performed to examine the practicability of prestressed strengthening of steel members using AC. A 3D digital image correlation (DIC) technique was used to monitor the bond behavior of prestressed CFRP to steel during prestress release and subsequent lap-shear tests. Experimental results of the current study revealed that AC of epoxy adhesive by heating can be a promising alternative to the conventional room temperature curing (RTC) for strengthening of steel members with externally bonded CFRP plates. It is evident that in prestressed strengthening projects, the prestressing system needs to stay in place to keep the prestressing force constant over the curing of the adhesive. Therefore, AC is certainly beneficial in reducing the required time and cost of such strengthening solutions. Based on the experimental results of the current study, a relatively fast practical solution for strengthening of existing steel members with prestressed bonded CFRP reinforcements is proposed.