000255551 001__ 255551
000255551 005__ 20180611214156.0
000255551 0247_ $$2doi$$a10.1038/s41467-018-04594-x
000255551 037__ $$aARTICLE
000255551 245__ $$aResolving molecule-specific information in dynamic lipid membrane processes with multi-resonant infrared metasurfaces
000255551 260__ $$c2018-06-04
000255551 269__ $$a2018-06-04
000255551 336__ $$aJournal Articles
000255551 520__ $$aA multitude of biological processes are enabled by complex interactions between lipid membranes and proteins. To understand such dynamic processes, it is crucial to differentiate the constituent biomolecular species and track their individual time evolution without invasive labels. Here, we present a label-free mid-infrared biosensor capable of distinguishing multiple analytes in heterogeneous biological samples with high sensitivity. Our technology leverages a multi-resonant metasurface to simultaneously enhance the different vibrational fingerprints of multiple biomolecules. By providing up to 1000-fold near-field intensity enhancement over both amide and methylene bands, our sensor resolves the interactions of lipid membranes with different polypeptides in real time. Significantly, we demonstrate that our label-free chemically specific sensor can analyze peptide-induced neurotransmitter cargo release from synaptic vesicle mimics. Our sensor opens up exciting possibilities for gaining new insights into biological processes such as signaling or transport in basic research as well as provides a valuable toolkit for bioanalytical and pharmaceutical applications.
000255551 700__ $$aRodrigo, Daniel
000255551 700__ $$aTittl, Andreas
000255551 700__ $$aAit-Bouziad, Nadine
000255551 700__ $$aJohn-Herpin, Aurelian
000255551 700__ $$aLimaj, Odeta
000255551 700__ $$aKelly, Christopher
000255551 700__ $$aYoo, Daehan
000255551 700__ $$aWittenberg, Nathan J.
000255551 700__ $$aOh, Sang-Hyun
000255551 700__ $$aLashuel, Hilal A.
000255551 700__ $$aAltug, Hatice
000255551 773__ $$j9$$k1$$q2160$$tNature Communications
000255551 8560_ $$fandreas.tittl@epfl.ch
000255551 909C0 $$0252159$$mhilal.lashuel@epfl.ch$$pLMNN$$xU11104
000255551 909C0 $$0252567$$mandreas.tittl@epfl.ch$$pBIOS$$xU12650
000255551 909CO $$ooai:infoscience.epfl.ch:255551$$particle$$pSTI$$pSV
000255551 960__ $$aandreas.tittl@epfl.ch
000255551 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000255551 980__ $$aARTICLE
000255551 981__ $$aoverwrite
000255551 981__ $$a255596