000254912 001__ 254912
000254912 005__ 20190317000945.0
000254912 0247_ $$2doi$$a10.1002/bit.26541
000254912 02470 $$2DOI$$a10.1002/bit.26541
000254912 037__ $$aARTICLE
000254912 245__ $$aConsolidated bioprocessing of lignocellulosic biomass to lactic acid by a synthetic fungal-bacterial consortium
000254912 260__ $$c2018
000254912 269__ $$a2018
000254912 336__ $$aJournal Articles
000254912 520__ $$aConsolidated bioprocessing (CBP) of lignocellulosic feedstocks to platform chemicals requires complex metabolic processes, which are commonly executed by single genetically engineered microorganisms. Alternatively, synthetic consortia can be employed to compartmentalize the required metabolic functions among different specialized microorganisms as demonstrated in this work for the direct production of lactic acid from lignocellulosic biomass. We composed an artificial cross‐kingdom consortium and co‐cultivated the aerobic fungus Trichoderma reesei for the secretion of cellulolytic enzymes with facultative anaerobic lactic acid bacteria. We engineered ecological niches to enable the formation of a spatially structured biofilm. Up to 34.7 gL−1 lactic acid could be produced from 5% (w/w) microcrystalline cellulose. Challenges in converting pretreated lignocellulosic biomass include the presence of inhibitors, the formation of acetic acid and carbon catabolite repression. In the CBP consortium hexoses and pentoses were simultaneously consumed and metabolic cross‐feeding enabled the in situ degradation of acetic acid. As a result, superior product purities were achieved and 19.8 gL−1 (85.2% of the theoretical maximum) of lactic acid could be produced from non‐detoxified steam‐pretreated beech wood. These results demonstrate the potential of consortium‐based CBP technologies for the production of high value chemicals from pretreated lignocellulosic biomass in a single step.
000254912 700__ $$aShahab, Robert L.
000254912 700__ $$aLuterbacher, Jeremy S.
000254912 700__ $$aBrethauer, Simone
000254912 700__ $$aStuder, Michael H.
000254912 773__ $$j115$$k5$$q1207-1215$$tBiotechnology and Bioengineering
000254912 8560_ $$fjherhau.yeap@epfl.ch
000254912 8564_ $$s104394$$uhttps://infoscience.epfl.ch/record/254912/files/Graphical%20Abstract.jpg$$zTHUMBNAIL
000254912 8564_ $$s1520083$$uhttps://infoscience.epfl.ch/record/254912/files/shahab_consolidated_2018_biotechnology%26bioengineering.pdf$$zFinal
000254912 8564_ $$s1969639$$uhttps://infoscience.epfl.ch/record/254912/files/shahab_consolidated_2018_biotechnology%26bioengineering.pdf?subformat=pdfa$$xpdfa$$zFinal
000254912 909C0 $$0252511$$mjherhau.yeap@epfl.ch$$pLPDC$$xU12907$$zBorel, Alain
000254912 909CO $$ooai:infoscience.epfl.ch:254912$$pSB$$particle$$qGLOBAL_SET
000254912 960__ $$ajherhau.yeap@epfl.ch
000254912 961__ $$amanon.velasco@epfl.ch
000254912 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000254912 981__ $$aoverwrite
000254912 980__ $$aARTICLE
000254912 980__ $$aWoS