Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplification in minimally buffered conditions producing, as a readout, pH shifts attaining subunits of pH.


Published in:
Chemical Communications, 54, 17, 2158-2161
Year:
Feb 02 2018
Laboratories:




 Record created 2018-03-08, last modified 2019-03-17

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