000253366 001__ 253366
000253366 005__ 20190507143837.0
000253366 0247_ $$a10.1186/s12951-018-0347-0$$2doi
000253366 037__ $$aARTICLE
000253366 245__ $$aNanostructured surface topographies have an effect on bactericidal activity
000253366 260__ $$c2018-02-28
000253366 269__ $$a2018-02-28
000253366 336__ $$aJournal Articles
000253366 520__ $$aBackground Due to the increased emergence of antimicrobial resistance, alternatives to minimize the usage of antibiotics become attractive solutions. Biophysical manipulation of material surface topography to prevent bacterial adhesion is one promising approach. To this end, it is essential to understand the relationship between surface topographical features and bactericidal properties in order to develop antibacterial surfaces. Results In this work a systematic study of topographical effects on bactericidal activity of nanostructured surfaces is presented. Nanostructured Ormostamp polymer surfaces are fabricated by nano-replication technology using nanoporous templates resulting in 80-nm diameter nanopillars. Six Ormostamp surfaces with nanopillar arrays of various nanopillar densities and heights are obtained by modifying the nanoporous template. The surface roughness ranges from 3.1 to 39.1 nm for the different pillar area parameters. A Gram-positive bacterium, Staphylococcus aureus, is used as the model bacterial strain. An average pillar density at ~ 40 pillars μm−2 with surface roughness of 39.1 nm possesses the highest bactericidal efficiency being close to 100% compared with 20% of the flat control samples. High density structures at ~ 70 pillars μm−2 and low density structures at < 20 pillars μm−2 with surface roughness smaller than 20 nm reduce the bactericidal efficiency to almost the level of the control samples. Conclusion The results obtained here suggests that the topographical effects including pillar density and pillar height inhomogeneity may have significant impacts on adhering pattern and stretching degree of bacterial cell membrane. A biophysical model is prepared to interpret the morphological changes of bacteria on these nanostructures.
000253366 6531_ $$aAntibacterial surface
000253366 6531_ $$aNanostructure
000253366 6531_ $$aNanoscale topography
000253366 6531_ $$aBactericidal activity
000253366 700__ $$g201009$$0245976$$aWu, Songmei
000253366 700__ $$aZuber, Flavia
000253366 700__ $$aManiura-Weber, Katharina
000253366 700__ $$aBrugger, Juergen
000253366 700__ $$aRen, Qun
000253366 773__ $$k1$$j16$$tJournal of Nanobiotechnology
000253366 8560_ $$fmohammadmahdi.kiaee@epfl.ch
000253366 909C0 $$mjuergen.brugger@epfl.ch$$pLMIS1$$0252040$$xU10321
000253366 909CO $$pSTI$$particle$$ooai:infoscience.epfl.ch:253366
000253366 960__ $$amohammadmahdi.kiaee@epfl.ch
000253366 961__ $$alaurence.gauvin@epfl.ch
000253366 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000253366 980__ $$aARTICLE
000253366 981__ $$aoverwrite