000233970 001__ 233970
000233970 005__ 20181203024925.0
000233970 0247_ $$2doi$$a10.1128/mBio.01283-17
000233970 022__ $$a2150-7511
000233970 02470 $$2ISI$$a000416271100045
000233970 037__ $$aARTICLE
000233970 245__ $$aInsights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
000233970 260__ $$bAmer Soc Microbiology$$c2017$$aWashington
000233970 269__ $$a2017
000233970 300__ $$a6
000233970 336__ $$aJournal Articles
000233970 520__ $$aMycobacterium lepraemurium is the causative agent of murine leprosy, a chronic, granulomatous disease similar to human leprosy. Due to the similar clinical manifestations of human and murine leprosy and the difficulty of growing both bacilli axenically, Mycobacterium leprae and M. lepraemurium were once thought to be closely related, although it was later suggested that M. lepraemurium might be related to Mycobacterium avium. In this study, the complete genome of M. lepraemurium was sequenced using a combination of PacBio and Illumina sequencing. Phylogenomic analyses confirmed that M. lepraemurium is a distinct species within the M. avium complex (MAC). The M. lepraemurium genome is 4.05 Mb in length, which is considerably smaller than other MAC genomes, and it comprises 2,682 functional genes and 1,139 pseudogenes, which indicates that M. lepraemurium has undergone genome reduction. An error-prone repair homologue of the DNA polymerase III alpha-subunit was found to be nonfunctional in M. lepraemurium, which might contribute to pseudogene formation due to the accumulation of mutations in nonessential genes. M. lepraemurium has retained the functionality of several genes thought to influence virulence among members of the MAC. IMPORTANCE Mycobacterium lepraemurium seems to be evolving toward a minimal set of genes required for an obligatory intracellular lifestyle within its host, a niche seldom adopted by most mycobacteria, as they are free-living. M. lepraemurium could be used as a model to elucidate functions of genes shared with other members of the MAC. Its reduced gene set can be exploited for studying the essentiality of genes in related pathogenic species, which might lead to discovery of common virulence factors or clarify host-pathogen interactions. M. lepraemurium can be cultivated in vitro only under specific conditions and even then with difficulty. Elucidating the metabolic (in) capabilities of M. lepraemurium will help develop suitable axenic media and facilitate genetic studies.
000233970 6531_ $$aMycobacterium lepraemurium
000233970 6531_ $$acomparative genomics
000233970 6531_ $$agenome sequencing
000233970 6531_ $$amurine leprosy
000233970 700__ $$0246601$$g229809$$uEcole Polytech Fed Lausanne, Global Hlth Inst, Lausanne, Switzerland$$aBenjak, Andrej
000233970 700__ $$uArizona State Univ, Sch Life Sci, Tempe, AZ USA$$aHonap, Tanvi P.
000233970 700__ $$0247821$$g241454$$uEcole Polytech Fed Lausanne, Global Hlth Inst, Lausanne, Switzerland$$aAvanzi, Charlotte
000233970 700__ $$uInst Nacl Psiquiatria Ramon de la Fuente, Dept Psicoinmunol, Mexico City, DF, Mexico$$aBecerril-Villanueva, Enrique
000233970 700__ $$uInst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico$$aEstrada-Garcia, Iris
000233970 700__ $$uInst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Inmunol, Mexico City, DF, Mexico$$aRojas-Espinosa, Oscar
000233970 700__ $$uArizona State Univ, Sch Human Evolut & Social Change, Tempe, AZ 85287 USA$$aStone, Anne C.
000233970 700__ $$0243892$$g177247$$uEcole Polytech Fed Lausanne, Global Hlth Inst, Lausanne, Switzerland$$aCole, Stewart T.
000233970 773__ $$j8$$tMbio$$k5$$qe01283-17
000233970 909C0 $$xU11742$$0252302$$pUPCOL
000233970 909CO $$pSV$$particle$$ooai:infoscience.tind.io:233970
000233970 917Z8 $$x282550
000233970 937__ $$aEPFL-ARTICLE-233970
000233970 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000233970 980__ $$aARTICLE