000233940 001__ 233940
000233940 005__ 20181203024924.0
000233940 0247_ $$2doi$$a10.1021/acssynbio.7b00088
000233940 022__ $$a2161-5063
000233940 02470 $$2ISI$$a000416204100001
000233940 037__ $$aARTICLE
000233940 245__ $$aA Microfluidic Biodisplay
000233940 260__ $$bAmerican Chemical Society$$c2017$$aWashington
000233940 269__ $$a2017
000233940 300__ $$a9
000233940 336__ $$aJournal Articles
000233940 520__ $$aSynthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves as an interface between an engineered cellular system, environment, and user. We created a biodisplay consisting of 768 individually programmable biopixels and demonstrated that it can perform multiplexed, continuous sampling. The biodisplay detected 10 mu g/L sodium-arsenite in tap water using a research grade fluorescent microscope, and reported arsenic contamination down to 20 mu g/L with an easy to interpret "skull and crossbones" symbol detectable with a low-cost USB microscope or by eye. The biodisplay was designed to prevent release of chemical or biological material to avoid environmental contamination. The microfluidic biodisplay thus provides a practical solution for the deployment and application of engineered cellular systems.
000233940 6531_ $$amicrofluidics
000233940 6531_ $$aenvironmental sensor
000233940 6531_ $$aheavy metal sensor
000233940 6531_ $$aarsenic
000233940 6531_ $$abiodisplay
000233940 6531_ $$acontinuous bacterial culturing
000233940 700__ $$0246680$$g227718$$aVolpetti, Francesca
000233940 700__ $$0247322$$g231804$$aPetrova, Ekaterina
000233940 700__ $$0243949$$g182445$$aMaerkl, Sebastian J.
000233940 773__ $$j6$$tAcs Synthetic Biology$$k11$$q1979-1987
000233940 909C0 $$xU11835$$0252240$$pLBNC
000233940 909CO $$pSTI$$particle$$ooai:infoscience.tind.io:233940
000233940 917Z8 $$x182445
000233940 937__ $$aEPFL-ARTICLE-233940
000233940 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000233940 980__ $$aARTICLE