Optical projection tomography for rapid whole mouse brain imaging

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 μm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer’s disease (AD). Finally, the OPT images are compared with histological slices.


Published in:
Biomedical Optics Express, 8, 12, 5637-5650
Year:
2017
Publisher:
Washington, Optical Soc Amer
ISSN:
2156-7085
Keywords:
Laboratories:


  • Histology slice of a 3-month-old 5xFAD mouse brain whose amyloid deposition has been labeled.: Visualization 5 - TIF; Histology slice of a 9-month-old B6SJL F1 mouse brain whose vasculature has been labeled.: Visualization 4 - TIF; Publisher's version: boe-8-12-5637 - PDF; Video of the 3D amyloid deposition in an 8-month-old 5xFAD mouse brain acquired with OPT.: Visualization 3 - AVI; Video of the 3D neuroanatomy of an 8-month-old B6SJL F1 mouse brain acquired with OPT.: Visualization 1 - AVI; Video of the 3D vascular network in a 4-month-old B6SJL F1 mouse brain acquired with OPT.: Visualization 2 - AVI; n/a: getThumbnail.cfm - JPG; URL
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 Record created 2017-11-16, last modified 2019-03-17

Histology slice of a 3-month-old 5xFAD mouse brain whose amyloid deposition has been labeled.:
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Histology slice of a 9-month-old B6SJL F1 mouse brain whose vasculature has been labeled.:
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Publisher's version:
Download fulltextPDF
Video of the 3D amyloid deposition in an 8-month-old 5xFAD mouse brain acquired with OPT.:
Download fulltextAVI
Video of the 3D neuroanatomy of an 8-month-old B6SJL F1 mouse brain acquired with OPT.:
Download fulltextAVI
Video of the 3D vascular network in a 4-month-old B6SJL F1 mouse brain acquired with OPT.:
Download fulltextAVI
n/a:
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